Research Associate Professor, IoG Associate Professor, Department of Human Genetics Medical School 4708 Med. Sci II Ann Arbor, MI 48109-0618 Tel: (734) 647-3149 Fax: (734) 763-3784 Email: dtburke@umich.edu

Dr. Burke's laboratory is analyzing the genetic basis of normal mammalian aging using the laboratory mouse as a model system. Several projects are in progress:

- An examination of the genetic and epigenetic mechanisms involved in maintenance of adult gene expression. The research measures the stability of genomic imprinting, X chromosome inactivation, and alternative splicing across the life span.

- The development of quantitative analysis systems for measuring DNA genotypes, RNA, and epigenetic DNA methylation. In collaboration with colleagues in the School of Engineering, we are developing silicon-fabricated miniaturized nucleic acid analysis systems.

- The design and implementation of experimental systems for the study of complex, multigenic phenotypes found primarily in late life. The research is performed in collaboration with colleagues at the University of Michigan Medical School, and uses reproducible populations of genetically characterized mice to study complex genetics and quantitative traits.

Publications:

Jackson AU, Galecki AT, Burke DT, Miller RA: Mouse loci associated with life span exhibit sex-specific and epistatic effects. J. Gerontol. Biological Sciences, 57A:B9-B15, 2002.

Miller RA, Chrisp C, Jackson AU, Galecki AT, Burke DT: Coordinated genetic control of neoplastic and non-neoplastic diseases in mice. J. Gerontol. Biological Sciences, 57A:B3-B8, 2002.

Ugaz VM, Brahmasandra SN, Burke DT, Burns MA: Cross-linked polyacrylamide gel electrophoresis of single-stranded DNA for microfabricated genomic analysis systems. Electrophoresis, 23:1450-1459, 2002.

Krishnan M, Brahmasandra SN, Burke DT, Mastrangelo CH, Burns MA: A novel strategy for the design of multiple reaction systems for genetic analysis. Sensors and Activators A, 95: 250-258, 2002.

Namasivayam V, Larson RG, Burke DT, Burns MA: Electrostretching DNA molecules using polymer-enhanced media within microfabricated devices. Analytical Chemistry, 74:3378-3385, 2002.

Ugaz VM, Burke DT, Burns MA: Microdevice-based measurements of diffusion and dispersion in cross-linked and linear polyacrylamide DNA sequencing gels. Electrophoresis, 23:2777-2787, 2002.

Miller RA, Harper JM, Galecki A, Burke DT: Big mice die young: early life body weight predicts longevity in genetically heterogeneous mice. Aging Cell, 1:22-29, 2002.

Jackson AU, Galecki AT, Burke DT, Miller RA: Genetic polymorphisms in mouse genes regulating age-sensitive and age-stable T cell subsets in mice. Genes and Immunity, 4:30-39, 2003.

Ugaz VM, Lin R, Srivastava N, Burke DT, Burns MA: A versatile microfabricated platform for electrophoresis of double- and single-stranded DNA. Electrophoresis, 24:151-157, 2003.

Volkman SK, Galecki AT, Burke DT, Paczas MR, Moalli MR, Miller RA, Goldstein SA: Quantitative trait loci for femoral size and shape in a genetically heterogeneous mouse population. J. Bone Miner. Res., 18:1497-1505, 2003.

Lin R, Burke DT, Burns MA: Selective extraction of size-fractionated DNA samples in microfabricated electrophoresis devices. J. Chromatogr. A. 1010:255-268, 2003.

Harper JM, Galecki AT, Burke DT, Pinkosky SL, Miller RA: Quantitative trait loci for insulin-like growth factor-I, leptin, thyroxine, and corticosterone in genetically heterogeneous mice, Physiol. Genomics, 15:44-51, 2003.

Bennett-Baker PE, Wilkowski J, Burke DT: Age-associated activation of epigenetically repressed genes in the mouse. Genetics 165:2055-2062, 2003.

Xu S, Yi N, Burke DT, Galecki AT, Miller RA: An EM algorithm for mapping binary disease loci: application to fibrosarcoma in a four-way cross mouse family. Genet. Res., 82:127-138, 2003 .

Namasivayam V, Larson RG, Burke DT, Burns MA: Light-induced molecular cutting: localized reaction on a single DNA molecule. Anal Chem.75:4188-4194, 2003.

Namasivayam V, Lin R, Johnson BN, Brahmasandra S, Razzacki Z, Burke DT, Burns MA: Advances in on-chip photodetection for applications in miniaturized genetic analysis systems. J. Micromech. Microeng. 14:81-90, 2004.

Harper JM, Galecki AT, Burke DT, Miller RA. Body weight, hormones and T-cell subsets as predictors of lifespan in genetically heterogeneous mice. Mech. Aging Devel. 125:381?390, 2004.

Volkman SK, Galecki AT, Burke DT, Miller RA, Goldstein SA: Quantitative trait loci that modulate femoral mechanical properties in a genetically heterogeneous mouse population. J. Bone Miner. Res. 19:1497-1505, 2004.

Wolf N, Galecki A, Lipman RD, Chen S, Burke DT, Miller RA. Quantitative trait locus mapping for age-related cataract severity and synechia prevalence using four-way cross mice. Invest. Ophth. Visual Sci., 45:1922-1929, 2004.

Lipman RD, Galecki AT, Burke DT, Miller RA: Genetic loci that influence cause of death in a heterogeneous mouse stock. J. Gerontol. (in press).

Pal R, Yang M, Johnson BN, Burke DT, Burns MA: Phase change microvalve for integrated devices. Analytical Chemistry. 76:3740-8, 2004.

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